control fvb n mice  (Charles River Laboratories)

Journal: The Journal of Clinical Investigation

Article Title: Activation of tyrosine kinase c-Abl contributes to α -synuclein–induced neurodegeneration

doi: 10.1172/JCI85456

Figure Lengend Snippet: (A, C, and E) Representative immunoblots of c-Abl, pY245 c-Abl, α-synuclein (α-syn), and β-actin in the spinal cord, brain stem, and cortex from symptomatic hA53Tα-syn transgenic mice and age-matched nontransgenic (Non-Tg) littermate controls. (B, D, and F) Quantification of pY245 c-Abl protein levels normalized to c-Abl (n = 4 per group). Data are from 3 independent experiments. Statistical significance was determined by 2-tailed unpaired Student’s t test. Quantified data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01. (G) Representative immunofluorescent images of pY245 c-Abl (green) and α-syn (red) in the brain stem and cortex from symptomatic hA53Tα-syn transgenic mice and age-matched nontransgenic littermate controls (n = 3 per group). Scale bar: 50 μm. (H) Representative confocal images of pY245 c-Abl (green) and pS129 α-syn (red) in the brain stem and cortex from symptomatic hA53Tα-syn transgenic mice and age-matched nontransgenic littermate controls (n =3 per group). Enlarged image (zoom-in, ×35; original magnification, ×40) at right shows colocalization of pY245 c-Abl and pS129 α-syn. Scale bar: 50 μm.

Article Snippet: Tetracycline-controllable BCR-ABL transgenic mice [FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J] were purchased from The Jackson Laboratory.

Techniques: Western Blot, Transgenic Assay

Journal: The Journal of Clinical Investigation

Article Title: Activation of tyrosine kinase c-Abl contributes to α -synuclein–induced neurodegeneration

doi: 10.1172/JCI85456

Figure Lengend Snippet: (A–C) Representative pS129 α-syn, ubiquitin, and GFAP IHC in the brain stem and cerebellum of 9-month-old symptomatic c-AblWT hA53Tα-syn mice versus c-AblKO hA53Tα-syn mice and age-matched littermate control mice (n = 3 per group). Enlarged images (zoom-in, ×30; original magnification, ×40) of the indicated regions are shown at right. Scale bars: 50 μm. (D) Representative immunoblots of c-Abl, α-syn, pS129 α-syn, and β-actin in the detergent-soluble fraction of brain stem from 9-month-old symptomatic c-AblWT hA53Tα-syn transgenic mice versus c-AblKO hA53Tα-syn transgenic mice and age-matched littermate controls. (E) Quantification of α-syn monomer and pS129 α-syn protein levels in D normalized to β-actin and α-syn monomer, respectively (n = 7 mice per group). (F) Representative immunoblots of c-Abl, α-syn, pS129 α-syn, and β-actin in the detergent-insoluble fraction of brain stem from 9-month-old symptomatic c-AblWT hA53Tα-syn transgenic mice and age-matched littermate controls. (G) Quantification of α-syn monomer and pS129 α-syn protein levels in F normalized to β-actin and α-syn monomer, respectively (n = 7 mice per group). (D–G) Data are from 3 independent experiments. Statistical significance was determined by 1-way ANOVA with Tukey’s post-test of multiple comparisons. Quantified data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01.

Article Snippet: Tetracycline-controllable BCR-ABL transgenic mice [FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J] were purchased from The Jackson Laboratory.

Techniques: Ubiquitin Proteomics, Control, Western Blot, Transgenic Assay

Journal: The Journal of Clinical Investigation

Article Title: Activation of tyrosine kinase c-Abl contributes to α -synuclein–induced neurodegeneration

doi: 10.1172/JCI85456

Figure Lengend Snippet: (A) Representative immunoblots of α-syn, pY39 α-syn, pS129 α-syn, pY245-c-Abl, c-Abl, and β-actin in the brain stem from nontransgenic mice of different ages. (B) Quantification of α-syn monomer and c-Abl protein levels in A normalized to β-actin (n = 5 mice per group). (C) Representative immunoblots of α-syn, pY39 α-syn, pS129 α-syn, pY245-c-Abl, c-Abl, and β-actin in the detergent-soluble fraction of brain stem from hA53Tα-syn transgenic mice of different ages. Asterisk indicates nonspecific band. (D) Quantification of pY245-c-Abl protein level normalized to c-Abl and pY39 α-syn and pS129 α-syn protein levels normalized to α-syn monomer in A (n = 5–10 mice per group). (E) Representative immunoblots of α-syn, pY39 α-syn, pS129 α-syn, pY245-c-Abl, c-Abl, and β-actin in the detergent-insoluble fraction of brain stem from hA53Tα-syn transgenic mice of different ages. (F and G) Quantification of pY245-c-Abl protein level normalized to c-Abl and pY39 α-syn and pS129 α-syn protein levels normalized to α-syn monomer in E (n = 5–10 mice per group). Data are from 3 independent experiments. Statistical significance was determined by 1-way ANOVA with Tukey’s post-test of multiple comparisons. Quantified data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Tetracycline-controllable BCR-ABL transgenic mice [FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J] were purchased from The Jackson Laboratory.

Techniques: Western Blot, Transgenic Assay